| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.191814 |
| Chromosome: | chromosome 9 |
| Location: | 6218818 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGTGCGGCCGTACGTGTACGTACGGAA |
| Internal bar code: | TACCCAATCAGACATTCTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 98.8685 |
| LEAP-Seq n confirming: | 699 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACCTAGCTACTGATCGC |
| Suggested primer 2: | GACGCAAGCATATGTCGCTA |