| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191838 |
| Chromosome: | chromosome 6 |
| Location: | 2688458 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270300 | (1 of 1) PTHR13271//PTHR13271:SF36 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGGCATCTACACTAGGTAGGCCAGCG |
| Internal bar code: | ACCCGCTTAAACGGGCACGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 992 |
| LEAP-Seq percent confirming: | 99.7142 |
| LEAP-Seq n confirming: | 2791 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGAGTCTTGTTGCTGTTG |
| Suggested primer 2: | ATAGGGCGACAAAACCCTCT |