Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.191848 |
Chromosome: | chromosome_6 |
Location: | 4068313 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278212 | CGL46 | Predicted protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGGATCCACTGTTCTCCGGATTTCGCCTC |
Internal bar code: | GCCTGCAAGGGGATTTTTGCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 597 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 94 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGGCTAAGCTTTCAATCGT |
Suggested primer 2: | TGTTCGAGTTCGTGCACTTC |