| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.191854 |
| Chromosome: | chromosome_17 |
| Location: | 4154014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g729850 | FAP227,C1A-18 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCGTTGGCGTGGATTTGGTGCGATGTGGC |
| Internal bar code: | GTTAGGTCCTTTGATAGTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 99.8811 |
| LEAP-Seq n confirming: | 1680 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTTGCCCTGGTACTTGT |
| Suggested primer 2: | CAAGCTGAGGATGGGGTAAA |