Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.191859 |
Chromosome: | chromosome_4 |
Location: | 373870 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g217914 | FAP57 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGCACACGTCCAAGCCGGTGATGGGGCCGG |
Internal bar code: | GATTGCTGCGGGGTCATAGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 280 |
LEAP-Seq percent confirming: | 99.5249 |
LEAP-Seq n confirming: | 7751 |
LEAP-Seq n nonconfirming: | 37 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTGTGTTCCATGTGAGGTG |
Suggested primer 2: | CCTGTCTTGCTTCGTGCATA |