Insertion junction: LMJ.RY0402.191886_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.191886
Chromosome:chromosome 13
Location:681790
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g566350 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCAATCCAAATCCACTGGTGACGCTCG
Internal bar code:CGGCCTCCATCGGTGACCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:448
LEAP-Seq percent confirming:86.3158
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGCATCATCAGCAGTGG
Suggested primer 2:GCACCTCAAACCTCCAACAT