| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191923 |
| Chromosome: | chromosome 8 |
| Location: | 2553956 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372450 | PSBQ1,OEE3, PSBQ | (1 of 1) K08901 - photosystem II oxygen-evolving enhancer protein 3 (psbQ); Photosystem II Oxygen Evolution Enhancer protein 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCATCTGTAAGGTCCAAAATCTCGCGCT |
| Internal bar code: | GCCCACCGACAGCTTCACCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 97.6079 |
| LEAP-Seq n confirming: | 1877 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTTAGTTGCGCATGAGG |
| Suggested primer 2: | GATGTGGCTTGTGTGTTTGG |