| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191953 |
| Chromosome: | chromosome 2 |
| Location: | 7236003 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145850 | FAP112 | Flagellar Associated Protein 112; (1 of 3) IPR006130 - Aspartate/ornithine carbamoyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACGCTGCGTGTTCGTCCAGAGCTCAGG |
| Internal bar code: | GTTGTGGGACGGCCGCCGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 92.395 |
| LEAP-Seq n confirming: | 814 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACTAGCGTGCCTTCAGC |
| Suggested primer 2: | GGTCTACCGCTGAAAGCAAG |