Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.191953 |
Chromosome: | chromosome_2 |
Location: | 7236239 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g145850 | FAP112 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGGATGCACTCCCAAATGGGTGTGCTATC |
Internal bar code: | GTGCCGGAGGGCAGGACTCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1074 |
LEAP-Seq percent confirming: | 99.4235 |
LEAP-Seq n confirming: | 3449 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTCTACCGCTGAAAGCAAG |
Suggested primer 2: | AGCTGCAACACGAAACACAC |