Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.191957 |
Chromosome: | chromosome_12 |
Location: | 3430776 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g496500 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGACCACCGAGTGGGTGGCCAGGCTGGCC |
Internal bar code: | GAATGCGTTCTTTTGGATAGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 981 |
LEAP-Seq percent confirming: | 99.8723 |
LEAP-Seq n confirming: | 1564 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAAAGGCTGAGGACAGTCGG |
Suggested primer 2: | CCAGTTGGTGTTTGATCACG |