| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191958 |
| Chromosome: | chromosome_2 |
| Location: | 7235093 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g145850 | FAP112 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCACAGCTGGTGACTTTACTATTGCCCAAC |
| Internal bar code: | GCCGTCGGCAGGACAGTGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.8055 |
| LEAP-Seq n confirming: | 3591 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTTCTTTTCGGGCACAG |
| Suggested primer 2: | ACAGCAAATGGTGCAACAAA |