Insertion junction: LMJ.RY0402.191969_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.191969
Chromosome:chromosome 3
Location:4890582
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g179450 SRR7 (1 of 1) K09624 - protease, serine, 12 (neurotrypsin, motopsin) [EC:3.4.21.-] (PRSS12); Scavenger receptor cysteine rich (SRCR) protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTCCCTGCCTGCTTGCCTACCTGTTTGC
Internal bar code:ACGCTATTAGCTGGATTCCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:492
LEAP-Seq percent confirming:99.569
LEAP-Seq n confirming:1386
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTAAATGTGTAGCCAGCGCA
Suggested primer 2:CGGGCAGGAGTAGAAGAGTG