| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191978 |
| Chromosome: | chromosome 17 |
| Location: | 3735834 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727400 | HRP8 | Hydroxyproline-rich glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGTCGGACGTCATGGTGGCGAGCGCTA |
| Internal bar code: | ACACATAATGGCGGCGGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 788 |
| LEAP-Seq percent confirming: | 99.7959 |
| LEAP-Seq n confirming: | 978 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACAGAACACTTCGGTCA |
| Suggested primer 2: | TCTCATAGTCCGGGTCCAAG |