| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191984 |
| Chromosome: | chromosome 4 |
| Location: | 3765517 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229518 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTAGGGTTTAGGGCCAAATCAGTGTCT |
| Internal bar code: | GAGAAAACACTGGTGTTAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 43.7399 |
| LEAP-Seq n confirming: | 17405 |
| LEAP-Seq n nonconfirming: | 22387 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACCG |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |