| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.191991 |
| Chromosome: | chromosome 12 |
| Location: | 1545634 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486950 | CSV7 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTGTGTGTGCGCGCGCCAGGATTGCGTT |
| Internal bar code: | CCCCATCGTGTGGTAGTCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1055 |
| LEAP-Seq percent confirming: | 99.0834 |
| LEAP-Seq n confirming: | 3351 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTTAGAGGAGCAGGTG |
| Suggested primer 2: | GTGCGAGCCTACACACGTTA |