| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.191995 |
| Chromosome: | chromosome 2 |
| Location: | 6773208 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118650 | PHC47 | Pherophorin-chlamydomonas homolog 47; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATCCTCATAGCCCACCATGCACACACC |
| Internal bar code: | GAGTTAGGTATAAGCGGGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 99.8534 |
| LEAP-Seq n confirming: | 681 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGACTCGCAGGAACAGGG |
| Suggested primer 2: | GCCGGTGTTGTGAAGTAGGT |