| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.191997 |
| Chromosome: | chromosome 5 |
| Location: | 234639 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233750 | (1 of 10) IPR029068 - Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAGCACTGAGTAGCTGTGGAAAGTGG |
| Internal bar code: | GGACTAGACTATTTTTATACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 99.5427 |
| LEAP-Seq n confirming: | 1959 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTTGCAGCTTTTGTGTG |
| Suggested primer 2: | ATGATTGCCGGACAAGAGAC |