| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192000 |
| Chromosome: | chromosome 6 |
| Location: | 4933622 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279550 | (1 of 62) PF00226 - DnaJ domain (DnaJ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGTGGGGGCATCAGGTTTCATGGTA |
| Internal bar code: | CTATGCGTTGGGTGCTCCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 99.487 |
| LEAP-Seq n confirming: | 6982 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCTTTATTTGGCGTTGT |
| Suggested primer 2: | GAGAGGTTGCTAGGGTGTCG |