| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192006 |
| Chromosome: | chromosome 3 |
| Location: | 1024813 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148400 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCCGCTGGTGGCGGTGCCGGTGGTGGC |
| Internal bar code: | CAACCTGTCCCTACGGTAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 24.4444 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAACACCCACCATAGCTG |
| Suggested primer 2: | GTCCTTCTTCTGCCAACAGC |