| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192033 |
| Chromosome: | chromosome 3 |
| Location: | 3133034 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165050 | PMA4 | (1 of 3) K01535 - H+-transporting ATPase (E3.6.3.6); Plasma membrane P-type calcium ATPase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGTAGATAGGGAGTTTCAGAGCACGCC |
| Internal bar code: | GAAGGGGGGACTGAGACGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 98.4475 |
| LEAP-Seq n confirming: | 2156 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCTTACTTCCTAGCCGC |
| Suggested primer 2: | GACGGCAAGGACGACTAGAG |