| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192053 |
| Chromosome: | chromosome 12 |
| Location: | 1787496 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512200 | MFT26 | Major facilitator superfamily transporter; (1 of 1) PTHR23504//PTHR23504:SF15 - FAMILY NOT NAMED // PROTEIN ZINC INDUCED FACILITATOR 1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCCCCCCCCCCCCTTTCGCTTCTTCTT |
| Internal bar code: | CACAGTGCAGCCGCGGCCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 99.5281 |
| LEAP-Seq n confirming: | 6327 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTCATCACGTCACCACC |
| Suggested primer 2: | CTACCCCGGTTGCATTCTTA |