| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192063 |
| Chromosome: | chromosome 16 |
| Location: | 2015649 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656900 | (1 of 77) IPR001623 - DnaJ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACGAGGCCCTGTGCGCTGGACGCGTGT |
| Internal bar code: | TGGGATTCCAATGCCGGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 95.9893 |
| LEAP-Seq n confirming: | 359 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACTGCATCCTCTGTTGC |
| Suggested primer 2: | ATACACCGGAGTGACTTGCC |