| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192070 |
| Chromosome: | chromosome 1 |
| Location: | 466100 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002700 | (1 of 1) PTHR13083//PTHR13083:SF3 - UNCHARACTERIZED // WD REPEAT-CONTAINING PROTEIN 91 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTAACAAACACCCCACCCCCACTTCAC |
| Internal bar code: | AGCTCAGAGAGCACTCACGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 87.875 |
| LEAP-Seq n confirming: | 2109 |
| LEAP-Seq n nonconfirming: | 291 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGGAGCTACACTTCTCTG |
| Suggested primer 2: | GCAGACAGATACGCAGGTCA |