Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.192082 |
Chromosome: | chromosome 13 |
Location: | 5157539 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g607700 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACTGCCATCTCTGTGTCATTATTGTAA |
Internal bar code: | CCATGCGTTGATCATGTGTCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 285 |
LEAP-Seq percent confirming: | 99.6612 |
LEAP-Seq n confirming: | 10297 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCTTGCAGTGATACGGACC |
Suggested primer 2: | GCCGACGAGTGGTATTGTTT |