| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.192090 |
| Chromosome: | chromosome_12 |
| Location: | 3981060 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAACGCAAAAATTTTTGGGGGTGCGGCGC |
| Internal bar code: | AGCGTTCGTCATTCGTTCCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 85.8407 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCAAGAACACCTGATCG |
| Suggested primer 2: | ATGGCTCATTGAACTTTGGG |