| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.192095 |
| Chromosome: | chromosome 2 |
| Location: | 1662206 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085650 | (1 of 1) K03424 - TatD DNase family protein (tatD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCGGCAACACCGAGCGAATGTTCTTTC |
| Internal bar code: | CTTAGGTCTAACTTGTTTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 797 |
| LEAP-Seq percent confirming: | 59.1667 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGGGGGTAGAATGTCA |
| Suggested primer 2: | GCTCTGGGGTAAGGGTAAGG |