| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192123 |
| Chromosome: | chromosome 1 |
| Location: | 5676885 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040450 | HDT1,HDPT1 | Histidine-aspartic acid phosphotransferase; (1 of 1) K14490 - histidine-containing phosphotransfer peotein (AHP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTTCAACGTATCATGGCAAACCAAGTT |
| Internal bar code: | GGGAAATCGCAAGACGACAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 986 |
| LEAP-Seq percent confirming: | 99.9243 |
| LEAP-Seq n confirming: | 3958 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGTACAAGGTGCTGAGG |
| Suggested primer 2: | ATAGTCGAACACAGCACCCC |