| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192156 |
| Chromosome: | chromosome_16 |
| Location: | 3629771 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g685573 | FAP214 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCATTTTAGAACATCGGGCCTTTCCCAGT |
| Internal bar code: | TTGATCTAGGATTCGCTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 74.731 |
| LEAP-Seq n confirming: | 2292 |
| LEAP-Seq n nonconfirming: | 775 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATCGACTTCTCTCGGC |
| Suggested primer 2: | CCATTCAGTGGTAGGCTGGT |