| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.192161 |
| Chromosome: | chromosome 10 |
| Location: | 1900063 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431850 | PGM18 | Phosphoglycerate mutase family protein; (1 of 1) PTHR23029//PTHR23029:SF49 - PHOSPHOGLYCERATE MUTASE // PHOSPHOHISTIDINE PHOSPHATASE SIXA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAACCTGTGCCGCCTGCTGCCGGCCTT |
| Internal bar code: | TGTTTACGGTAGAGGCGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 99.2527 |
| LEAP-Seq n confirming: | 2922 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACACGACTAGCCCACGG |
| Suggested primer 2: | CTTGGCAACCTGTGTGTTTG |