| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.192163 |
| Chromosome: | chromosome 12 |
| Location: | 3039954 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500650 | RNB2 | (1 of 1) PTHR23355//PTHR23355:SF39 - RIBONUCLEASE // SUBFAMILY NOT NAMED; chloroplast RNase II | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATAATCTAGGTCCGAGCGTGCAAGTAA |
| Internal bar code: | TCCGATTATCGTCTCGTCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 99.6241 |
| LEAP-Seq n confirming: | 1060 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGACTGATGACTGTGCT |
| Suggested primer 2: | GGTCGCTTTGGTGTACGATT |