| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192176 |
| Chromosome: | chromosome_12 |
| Location: | 7504026 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g555950 | DHC11 | Putative flagellar inner arm dynein heavy chain, novel minor typ | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGACCTGTCCAAACTGTTCCAGGTGCGGT |
| Internal bar code: | CACGTCGCCGAGGGTCATCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 99.7855 |
| LEAP-Seq n confirming: | 4186 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGTGCATTGTGCTCTCC |
| Suggested primer 2: | CCTCACTCCTCAACTCCAGC |