Insertion junction: LMJ.RY0402.192176_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.192176
Chromosome:chromosome_12
Location:7506918
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g555950 DHC11 Putative flagellar inner arm dynein heavy chain, novel minor typ antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GGTGACCCGTGCCGGCAGGGCGGCCAGCCA
Internal bar code:TCATGCTAACCGTGTGAGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1001
LEAP-Seq percent confirming:99.3939
LEAP-Seq n confirming:492
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGATTTCAACTGCCCACC
Suggested primer 2:TGGACGACAACAAGAAGCTG