Insertion junction: LMJ.RY0402.192190_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.192190
Chromosome:chromosome 3
Location:3187698
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g165300 (1 of 1) PF13350 - Tyrosine phosphatase family (Y_phosphatase3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGAAAACACACACACACACACACGCCT
Internal bar code:CCGTGGGGTATCGATCTAATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:806
LEAP-Seq percent confirming:95.8656
LEAP-Seq n confirming:4336
LEAP-Seq n nonconfirming:187
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCATTCTCCCTATACCGT
Suggested primer 2:AAATGCCGACCTACAACTGG