| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192207 |
| Chromosome: | chromosome 14 |
| Location: | 3758905 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632300 | HDA6 | (1 of 2) PTHR10625//PTHR10625:SF131 - HISTONE DEACETYLASE // SUBFAMILY NOT NAMED; RPD3/HDA1 type histone deacetylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTATACAGATTAAGAAGCATCCGGTTC |
| Internal bar code: | CCCCGCCTTCTCGCCCCGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 98.4799 |
| LEAP-Seq n confirming: | 4211 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGTTGCAGGGGGTAGCA |
| Suggested primer 2: | ACAGACAACATTTAGGCCCG |