| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192213 |
| Chromosome: | chromosome 9 |
| Location: | 7242370 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412550 | (1 of 3) IPR000104//IPR005069 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAAACCGCTGGACCCTCCCCTGGCTTCC |
| Internal bar code: | CCGTGTCCTGGGGAAGCAGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 99.5327 |
| LEAP-Seq n confirming: | 426 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCGACTCACGATGCTA |
| Suggested primer 2: | GCGTGTGGTGATTTTGTTTG |