Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.192220 |
Chromosome: | chromosome 7 |
Location: | 2620006 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g330300 | (1 of 2) K14411 - RNA-binding protein Musashi (MSI) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTATGCAACATGACAGACAAACAAAGCGC |
Internal bar code: | CGCGCGTAACGAGCGCTGACGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 666 |
LEAP-Seq percent confirming: | 99.8107 |
LEAP-Seq n confirming: | 2636 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGGGAGAGTTCGAGACTGG |
Suggested primer 2: | TCTGCAATGGTCTGAACTGC |