| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.192220 |
| Chromosome: | chromosome 7 |
| Location: | 2620043 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330300 | (1 of 2) K14411 - RNA-binding protein Musashi (MSI) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAGAGGACCGGTCAGTCTTTGCGGGGG |
| Internal bar code: | CCACTGTTGATCTGGAAAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 98.3681 |
| LEAP-Seq n confirming: | 1085 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGAGAGTTCGAGACTGG |
| Suggested primer 2: | TCTGCAATGGTCTGAACTGC |