Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.192222 |
Chromosome: | chromosome_17 |
Location: | 1628742 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g708000 | FAP260 | Flagellar Associated Protein, membrane-associated | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTTAGTGTCAATGGCCCATCCTGAAGAGA |
Internal bar code: | GTGACGCCCTAAAATAGCTGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 683 |
LEAP-Seq percent confirming: | 69.0941 |
LEAP-Seq n confirming: | 3188 |
LEAP-Seq n nonconfirming: | 1426 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGGCACGAGCCGTTTAGTA |
Suggested primer 2: | GAGCCAACGACCTATTGGAA |