Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.192224 |
Chromosome: | chromosome 11 |
Location: | 536853 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467603 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCGCAATGCCCCCATGCGCAAGCAAAC |
Internal bar code: | GGCGGTACTCTGGCCCGAGCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 693 |
LEAP-Seq percent confirming: | 99.3435 |
LEAP-Seq n confirming: | 454 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCACTACCCACGAGAACC |
Suggested primer 2: | ATACACGCACCCACAGCATA |