| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192248 |
| Chromosome: | chromosome 12 |
| Location: | 5175184 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527500 | (1 of 1) K04508 - transducin (beta)-like 1 (TBL1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGCAGGGCGGTGCAAGCTGGCTGTGG |
| Internal bar code: | AATTACCCCTCGCTATCGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 88.5906 |
| LEAP-Seq n confirming: | 132 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGCCAGTACAATCAACTT |
| Suggested primer 2: | GGATATGGACCAAGGAGGGT |