| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.192312 |
| Chromosome: | chromosome 8 |
| Location: | 2163225 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369350 | (1 of 2) 2.6.1.52 - Phosphoserine transaminase / PSAT | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGATCGGGCACGTGCGTGGTCAAAGGAC |
| Internal bar code: | TCTCCGCTGGGCCCCGTGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 99.4326 |
| LEAP-Seq n confirming: | 1402 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAACAGGTCACTCTGCAA |
| Suggested primer 2: | CAGCCATGATGGTGTAGGTG |