| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192322 |
| Chromosome: | chromosome 12 |
| Location: | 2678930 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504000 | (1 of 2) PTHR12121:SF34 - LD39302P | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTCCTCAGTCCTCACTACCGCCCCAGC |
| Internal bar code: | GTGAGGATGAAGTAGCTTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 22.6567 |
| LEAP-Seq n confirming: | 817 |
| LEAP-Seq n nonconfirming: | 2789 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTTGCCGTGATTTCAT |
| Suggested primer 2: | GGGGCTTTGTCACATCTGTT |