| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192376 |
| Chromosome: | chromosome 3 |
| Location: | 3932120 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171461 | HEMN1 | Coproporphyrinogen III oxidase; (1 of 1) 1.3.99.22 - Coproporphyrinogen dehydrogenase / Oxygen-independent coproporphyrinogen-III oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGCCCTGGCTTGGCGTAGTTGCTGACC |
| Internal bar code: | TTAAGCTTTGGGGCGGTCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 288 |
| LEAP-Seq percent confirming: | 99.7895 |
| LEAP-Seq n confirming: | 948 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTCCGTTTGGGTAGACT |
| Suggested primer 2: | ACACCTGGAGGGTGTGGTAG |