Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.192380 |
Chromosome: | chromosome_6 |
Location: | 6499928 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g293100 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTCATACTTTGAAGGGCACAGACTGGCAG |
Internal bar code: | GAGGCTGAAGGGGCCTTGGTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1044 |
LEAP-Seq percent confirming: | 92.6756 |
LEAP-Seq n confirming: | 1860 |
LEAP-Seq n nonconfirming: | 147 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTGTTGAAATGCTTTGCT |
Suggested primer 2: | GGGCTAGCTCAGTCAAGGTG |