| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192380 |
| Chromosome: | chromosome 12 |
| Location: | 1309848 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488850 | AP2A1 | Alpha2-Adaptin; (1 of 1) K11824 - AP-2 complex subunit alpha (AP2A) | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCACACCCGCGACCTCCTTCAAAACGCCC |
| Internal bar code: | GAGGTTAAGCCTCCGCATTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 99.7636 |
| LEAP-Seq n confirming: | 1266 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGACCTGCTGTCCATGA |
| Suggested primer 2: | TGCAAACAAAAGCAAAGCAC |