Insertion junction: LMJ.RY0402.192392_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.192392
Chromosome:chromosome 2
Location:4805347
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g102850 CPZ (1 of 1) IPR000834//IPR016024 - Peptidase M14, carboxypeptidase A // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCATCGCCAGCCCCCGCGGCCTCGTGA
Internal bar code:GGGGGCGTCCGTGGTGGCATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:751
LEAP-Seq percent confirming:98.8889
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTTCACGACAGCTTGGA
Suggested primer 2:AAAGCAGGAGTGGAAGCTGA