| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192402 |
| Chromosome: | chromosome 17 |
| Location: | 3345861 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723450 | FAP13 | WD-repeat Flagellar Associated Protein 13; (1 of 1) IPR001680//IPR007111//IPR017986//IPR020472//IPR027417 - WD40 repeat // NACHT nucleoside triphosphatase // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATGGGGCAGCAGAGGACGGGGAAGGCG |
| Internal bar code: | CCGCTATAGAGACAGCGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 99.7236 |
| LEAP-Seq n confirming: | 5773 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAGCCTGCAATGTCGCTT |
| Suggested primer 2: | ACCCAAGCTCACCACAATTC |