| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192428 |
| Chromosome: | chromosome 6 |
| Location: | 1843575 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262850 | RAB23 | Small Rab-related GTPase; (1 of 1) K06234 - Ras-related protein Rab-23 (RAB23) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTTCGGATGATGTGTTAGTGCACGTGC |
| Internal bar code: | GAGGCACCAGAACGGGTTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 98.7233 |
| LEAP-Seq n confirming: | 3557 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCTGCACCATAAGAAGC |
| Suggested primer 2: | TTACGTCTACCTGCCCATCC |