Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.192429 |
Chromosome: | chromosome_12 |
Location: | 2699158 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g503800 | RMT5 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCAGCTCCGCCGCCACCGCTTTCCAGCCTC |
Internal bar code: | GGAAAAAGAGTATCGTGGAGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 557 |
LEAP-Seq percent confirming: | 98.0576 |
LEAP-Seq n confirming: | 1565 |
LEAP-Seq n nonconfirming: | 31 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTGTGCTGGGTAGGTAGGT |
Suggested primer 2: | CATATCATAATGGCCCCCAC |