Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.192454 |
Chromosome: | chromosome 14 |
Location: | 1372033 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g617200 | RLS7 | RegA/Rls-like protein | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCCACTGACCCACTCATTGAACCTCCC |
Internal bar code: | TCTGGCCACACGCGGTGCTTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1121 |
LEAP-Seq percent confirming: | 99.0991 |
LEAP-Seq n confirming: | 440 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAGTATCAAAACGGCCACT |
Suggested primer 2: | TAGCCTGATCAGTGCGTGTC |